Sigma-Aldrich Photochromic Trpa1 Channel

Assay

≥95% (HPLC)

Quality Level

100

Form

powder

Potency

2 μM EC₅₀

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
protect from light

Color

yellow

Solubility

DMSO: 100 mg/mL

Storage Temp.

2-8°C

General Description

A rhodanine derivative that acts as a reversible, photoactivated (λ_{max = 415 nm), and highly TrpA1-selective channel activator, presumably by a light-activated cysteine alkylation mechanism via the α,β-unsaturated alkene group of Optovin rhodanine moiety, and enables light-induced primary murine DRG calcium response in a TrpA1-dependent and HC-030031- (Cat. No. 648485) blockable manner, allowing light-induced activation only in hTrpA1-expressing, but not TrpV1- or TrpM8-expressing, HEK293T transfectants. Itsin vivoefficacy is demonstrated in light-induced zebrafish motor activity (EC50/ECmax= 2 µM/3 µM; with >1.6 µW/min 387-405 nm excitation) and low power 405 nm laser-induced head shake/twitch response in mice (0.3 µmol Optovin/20 uL DMSO/ear applied atopically before laser illumination). Exhibits little or no inhibitory activity toward hERG or a panel of 50 human and rodent CNS receptors, channels, and transporters.}A rhodanine derivative that acts as a reversible, photoactivated (λ_max= 415 nm), and highly TrpA1-selective channel activator and enables light-induced primary murine DRG calcium response in a TrpA1-dependent and HC-030031- (Cat. No. 648485) blockable manner, allowing light-induced activation only in hTrpA1-expressing, but not TrpV1- or TrpM8-expressing, HEK293T transfectants. Itsin vivoefficacy is demonstrated in light-induced zebrafish motor activity (EC₅₀/EC_max= 2 µM/3 µM) and low power 405 nm laser-induced head twitch response in mice (0.3 µmol Optovin/20 uL DMSO/ear applied atopically). Exhibits little or no inhibitory activity toward hERG or a panel of 50 human and rodent CNS receptors, channels, and transporters.

Biochem/Physiol Actions

Primary Target
TrpA1

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Other Notes

Kokel, D., et al. 2013,Nat. Chem. Biol. 9,257.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany