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GenScript Gencrispr Sgrna Screening Kit; 30reactions
List Price
$166.00
Your Price
$166.00
GenScript Gencrispr Sgrna Screening Kit30reactions - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-L00689-30
MFG.PART:
L00689-30
UNSPSC:
41105505
Manufacturer:
GenScript USA Inc
Description
Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA.
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Note
Research purposes only.
Product Introduction
| Storage | 1. The positive control sgRNA is provided as a freeze-dried powder that should be stored at -80°C once received. It is recommended to be used within 3 months after receiving. Redissolve with RNase-free water before using. 2. The activity of the positive control sgRNA will decrease after free-thaw cycles. Please aliquot and store at-80°C, to avoid repeated freeze/thaw cycles. 3. There commended storage temperature of GenCrispr Cas9 Nuclease and 10X Reaction buffer is -20°C. |
Applications
| Evaluate the efficiency of sgRNAs for CRISPR/Cas9 in vitro studies and identify the highly effective sgRNAs before cell transfection. |
| SKU | GSCRPT-L00689-30 |
|---|---|
| Supplier Part Number | L00689-30 |
| UM | EA |
| UNSPSC | 41105505 |
| Manufacturer | GenScript USA Inc |
| Temperature | 1. The positive control sgRNA is provided as a freeze-dried powder that should be stored at -80°C once received. It is recommended to be used within 3 months after receiving. Redissolve with RNase-free water before using. 2. The activity of the positive c |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |