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GenScript T7 Rna Polymerase; 10kU
List Price
$120.00
Your Price
$120.00
GenScript T7 Rna Polymerase10ku - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-E00066-10
MFG.PART:
E00066-10
UNSPSC:
12352204
Manufacturer:
GenScript USA Inc
Description
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5→ 3 direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications. |
Note
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Properties
| Source | Recombinant T7 RNA Polymerase expressed by E.coli |
| Species | phage T7 |
| Molecular Weight | ~100 kDa |
| Unit Definition | One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C. |
| Optimal active temperature | 37 °C. |
| Storage Buffer | This enzyme is supplied in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 10 mM DTT, 50% Glycerol, 0.1% Triton X-100, pH 8.0. |
| Storage & Stability | This product remains stable for up to 12 months at -20 °C. Avoid repeated freeze-thaw cycles. |
Applications
| · Synthesis of the single-strand RNA |
Quality Control Specifications
| Appearance | Clear, colorless liquid |
| Purity | ≥ 95% as analyzed by SDS-PAGE |
| Enzyme Activity | ≥ 50 U/μl |
| Endotoxin Level | ≤ 0.1 EU/µg of protein as analyzed by gel clotting method |
| Residual Endonuclease | Non-detectable |
| Residual Exonuclease | Non-detectable |
| Residual RNase | Non-detectable |
Background
| References | 1. J Kochetkov, S. N., E. E. Rusakova, and V. L. Tunitskaya. "Recent studies of T7 RNA polymerase mechanism." FEBS letters 440.3 (1998): 264-267. |
| SKU | GSCRPT-E00066-10 |
|---|---|
| Supplier Part Number | E00066-10 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | This product remains stable for up to 12 months at -20 °C. Avoid repeated freeze-thaw cycles. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |