Takara pTet-Off Advanced Vector \20 ug

Takara The pTet-Off Advanced Vector is used to create stable Tet-Off Advanced cell lines as hosts for a Tet-inducible expression system. The Tet-Off Advanced protein is a modified Tet-responsive transactivator (tTA-Advanced) that binds tightly to tetracycline-responsive elements (TRE) and strongly activates transcription of a downstream gene in the absence of tetracycline (Tc) or doxycycline (Dox; a Tc derivative). As Tc or Dox is added to the culture medium, transcription from the TRE or TRE-Tight is turned off in a highly dose-dependent manner.The Tet-Off Advanced gene is fully synthetic and has been optimized for expression and stability in mammalian cells by incorporating mammalian codon preferences and by removing cryptic splice sites from the mRNA. In addition, 3 minimal "F-type" HSV VP16 transcription activation domains provide maximal activation and reduced toxicity. The immediate early promoter of cytomegalovirus (PCMV IE) produces strong constitutive expression of Tet-Off Advanced, which accumulates to high levels. Stable Tet-Off Advanced cell lines can be generated by transfecting the plasmid and selecting for G418-resistant clones.- TAKBGN (Additional S&H or Hazmat Fees May Apply)