Takara Lenti-X SARS-CoV-2 Packaging Single Shots (D614G Spike, Truncated) \12 Rxns

Takara Lenti-X SARS-CoV-2 Packaging Single Shots (D614G Spike, Truncated) enable streamlined production of high-titer lentivirus pseudotyped with a truncated form of the spike protein from SARS-CoV-2 (D614G mutant) by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involves reconstituting a tube of lyophilized packaging mix with a lentiviral transfer vector of choice in sterile water and adding the mixture to 293T cells (e.g., Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.Each kit includes 12 tubes of Lenti-X SARS-CoV-2 Packaging Mix (D614G Spike, Truncated; Cat. # 632675), 6 tubes of Lenti-X SARS-CoV-2 Packaging Mix (No-Envelope Control; Cat. # 632676) for production of pseudoviral particles lacking an envelope protein, and the Lenti-X SARS-CoV-2 Vector Set (Cat. # 632677), which includes two self-inactivating lentiviral transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction.For your convenience, the packaging mixes included in the kit are also sold separately. - TAKBGN (Additional S&H or Hazmat Fees May Apply)