Genscript AmMag Ultra AT protein A magbeads

Intended Use
AmMag™ Ultra AT protein A magbeadsare developed and optimizedfor antibody purification for high throughput and high volume applications.

Principle
The sample containingantibody is added to the AmMag™ Ultra AT protein Amagbeads and incubated on a rotor overnight or 2 hours (depending on theconcentration of samples) for the antibody to bind to the beads. The antibodybound to the beads can be eluted off by using acidic elution facilitated by amagnetic separation device. Magnetic separation eliminates the need forcentrifugation, minimizes the loss of sample and makes the process more userfriendly.

Material Supplied
GenScript AmMag™ Ultra AT protein A magbeads are super paramagneticbeads with diameter of 45-100 μm, covalently coated with Ultra alkalinetolerant protein A, After treatment with 1M NaOH for 24h, the loaddecreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol.The AmMag™ Ultra AT protein Amagbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. Theycan be reused for up to 100 cycles. After 100 cycles, there was almost no decrease inbinding load.
Protein A, a bacterial cellwall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s,mainly through the Fc regions. The alkaline tolerant protein A not only keepsthe specific binding capacity to Fc region of immunoglobulin molecules, butalso tolerates alkaline conditions and can withstand rigorous cleaningprocedures.