Miltenyi Biotec Macsprep Cd19 Car Microbead Kit, Human; For 100 mL whole blood

Miltenyi Biotec The MACSprep CD19 CAR MicroBead Kit, human has been developed for the fast isolation of CD19 CAR cells from freshly drawn anticoagulated whole blood without density gradient centrifugation nor red blood cell (RBC) lysis. During the first isolation step RBCs are aggregated and sedimented. In the second step, CD19 CAR cells are labeled with CD19 CAR Detection Reagent, human, Biotin. After one washing step the CD19 CAR Detection Reagent-bound cells are magnetically labeled with Anti-Biotin MicroBeads. Then, the cell suspension is separated by loading it onto a MACS Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD19 CAR cells are retained within the column. The unlabeled cells run through, this cell fraction is thus depleted of CD19 CAR cells. After removing the column from the magnetic field, the magnetically retained CD19 CAR cells can be eluted as the positively selected cell fraction. | - MLTY (Additional S&H or Hazmat Fees May Apply)