Sigma-Aldrich SMC Interleukin 23 (IL-23) Immunoassay Kit

Interleukin-23 (IL-23) is an endogenous cytokine responsible for the production of IL-17 from memory T-lymphocytes. The active human form of IL-23 is heterodimeric, consisting of a p40 subunit shared with IL-12, and a p19 subunit unique to IL-23. The p19 subunit is expressed by T-lymphocytes, activated macrophages, dendritic and endothelial cells. IL-23 also promotes upregulation of MMP-9 and the stimulation of helper T-lymphocytes to Th17 cells. The proinflammatory characteristics of IL-23 make it a target of interest for a number of Th17 autoimmune disorders, including Crohn′s disease, colitis, inflammatory bowel disease, and psoriasis.

The SMC^® IL-23 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Human IL-23 Immunoassay Kit unparalleled performance in the quantification of IL-23 in human EDTA plasma and serum.

Used to detect/quantify:IL-23

Assay Buffer
Human IL-23 Coated Beads
Standard Diluent
Human IL-23 Detection Antibody
Human IL-23 Standard
SMC^® Human IL-23 Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D

Low-end CV% Range: 0-13%|Low-end CV% Average: 6%

SMC is a registered trademark of Singulex

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial