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GenScript Benz-Neburase™ Gmp, Tag Free; 10kU
List Price
$75.00
Your Price
$75.00
GenScript Benz-Neburase™ Gmp, Tag Free - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-Z03708-10
MFG.PART:
Z03708-10
UNSPSC:
12352204
Manufacturer:
GenScript USA Inc
Overview
| Product Introduction | Benz-Neburase™ GMP, tag-free is a genetically engineered endonuclease from Serratia marcescens that cleaves all forms of DNA and RNA non-specifically under a broad range of conditions. Benz-Neburase™ Nuclease is mostly utilized to remove residual DNA and RNA during production process of biological molecules of interest in biopharmaceutical manufacturing including but not limited to vaccine production, viral vector production, as well as in gene and cell therapy-related manufacturing. |
Properties
| Expression System | E.coli |
| Species | Serratia marcescens |
| Tag | Tag free |
| Theoretical Molecular Weight | 27.0 kDa |
| Biological Activity | ≥ 250 U/μl (or 1.1 × 106 U/mg) One unit is defined as the amount of enzyme that digests sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (within reaction volume 0.5 ml). |
| Storage Buffer | 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
| Storage & Stability | Store at -20 °C for up to 24 months or at 4 °C for 2 weeks from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Applications
| Digests all forms of DNA and RNA (double stranded, single stranded, linearized, and circular forms) under a board range of conditions. |
Quality Control Specifications
| Appearance | Clear, colorless liquid |
| Purity | SEC-HPLC: ≥ 99% Reducing SDS-PAGE: ≥ 95% |
| Endotoxin Level | < 0.01 EU/μg |
| Residual HCP | ≤ 20 ng/mg |
| Protease Activity | Non-detectable |
| Bioburden | < 1 CFU/ml |
| Mycoplasma | Negative |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03708-10 |
|---|---|
| Supplier Part Number | Z03708-10 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| MSDS URL | Click here |
| Temperature | Store at -20 °C for up to 24 months or at 4 °C for 2 weeks from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |