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GenScript Gencrispr Ercas12a Nuclease; 100mg
List Price
$189.00
Your Price
$189.00
GenScript Gencrispr™ Ercas12a Nuclease Is An Rna-Guided Dna Endonuclease From Eubacterium Rectale. Cas12a (Previously Known As Cpf1) Belongs To The Class 2 Type V Crispr/Cas System - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-Z03762-100
MFG.PART:
Z03762-100
UNSPSC:
12352204
Manufacturer:
GenScript USA Inc
Overview
| Description | GenCRISPR™ ErCas12a Nuclease is an RNA-guided DNA endonuclease from Eubacterium rectale. Cas12a (previously known as Cpf1) belongs to the Class 2 Type V CRISPR/Cas system. Different from CRISPR/Cas9 system, the ribonucleoprotein (RNP) complex of CRISPR/Cas12a system is formed by Cas12a and crRNA, it recognizes a T-rich protospacer adjacent motif (PAM) and results in a staggered DNA double-strand break (DSB). After the specific cleavage, Cas12a can also activate collateral cleavage activity towards adjacent non-specific ssDNA sequences. Hence, Cas12a nuclease is a good alternative for Cas9 in certain target DNA editing, and provides a novel strategy for DNA detection. |
Applications
| crRNA-dependent DNA cleavage |
Properties
| Source | Recombinant ErCas12a with a N-terminal NLS expressed by E.coli |
| Species | Eubacterium rectale |
| Tag | C-terminal 8× His Tag |
| Theoretical Molecular Weight | ~148 kDa |
| Concentration | 4 mg/ml |
| Storage Buffer | 20 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.4 |
| Storage & Stability | Store at -20 °C for up to 18 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| Accession# | WP_055225123.1 |
Quality Control Specifications
| Appearance | Clear, colorless liquid |
| Purity | ≥ 90% (by SDS-PAGE) |
| Concentration by A280 | 4 (± 10%) mg/ml |
| Bioactivity (in vitro) | ≥ 90% |
| Residual DNase | Undetectable |
| Residual RNase | Undetectable |
| Endotoxin Level | < 0.1 EU/μg |
Background
| References | 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system." Cell 163.3 (2015): 759-771. 2. Liu, Zhenyi, et al. "ErCas12a CRISPR-MAD7 for model generation in human cells, mice, and rats." The CRISPR journal 3.2 (2020): 97-108.Chen, Janice S., et al. "CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity." Science 360.6387 (2018): 436-439. 3. Rojek, Johan, et al. "Mad7: An IP friendly CRISPR enzyme." Authorea Preprints (2021). |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03762-100 |
|---|---|
| Supplier Part Number | Z03762-100 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store at -20 °C for up to 18 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |