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GenScript Benz-Neburase™, Tag-Free; 10kU
List Price
$49.00
Your Price
$49.00
GenScript Benz-Neburase™, Tag-Free - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-Z03695-10
MFG.PART:
Z03695-10
UNSPSC:
12352204
Manufacturer:
GenScript USA Inc
Properties
| Expression System | E.coli |
| Species | Serratia Marcescen |
| Tag | Tag-free |
| Theoretical Molecular Weight | 27.5 kDa |
| Biological Activity | ≥ 1.1×106 U/mg One unit of Benz-Neburase, tag-free is defined as the amount of enzyme for a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30 min. |
| Formulation | Supplied as a solution of 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
| Apparent Molecular Weight | ~27.5 kDa, on SDS-PAGE under reducing conditions |
| Storage & Stability | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Applications
| The Benz-Neburase™ is an endonuclease capable of removing all forms of DNA and RNA, including double stranded, single stranded, linearized, and circular forms. The Benz-Neburase is commonly used in biopharmaceutical production such as vaccine, viral vector, gene and cell therapy manufacturing facilities. | |
| The activity of Benz-Neburase™, tag-free requires 1-2 mM Mg2+. |
Quality Control Specifications
| Purity | ≥ 95% as analyzed by reducing SDS-PAGE |
| Enzyme Activity | ≥ 250 U/μL |
| Endotoxin Level | ≤ 0.1 EU/kU as determined by gel clotting method. |
Background
| Target Background | The Benz-Neburase is a genetically engineered endonuclease from Serratia marcescens used as a DNA eraser in the purification processes of biological molecules. The enzyme cleaves all forms of DNA and RNA into smaller nucleotides of around 5-8 base pairs. Benz-Neburase requires divalent cation, preferably Mg2+ for activity, displays a broad pH tolerance, ranging from pH 6 to pH 10, with an optimal pH of 8-8.5, and has a wide temperature tolerance, ranging from 35 °C to 44 °C. The nuclease is a physiologic homodimer and functions more progressively than the monomer. Two disulfide bonds in the nuclease are crucial to its activity and stability. The enzyme is active in a broad range of conditions and is free of proteolytic activity. This makes the enzyme especially useful for biopharmaceutical applications with contaminating DNA residue, such as lysed host cells in viral vector manufacturing processes. |
| Synonyms | alternative to Benzonase® Benzonase is a registered trademark of Merck KGaA |
| References | 1. Nestle, Marion, and W. K. Roberts. "An extracellular nuclease from Serratia marcescens: I. Purification and some properties of the enzyme." Journal of Biological Chemistry 244.19 (1969): 5213-5218. 2. Benedik, Michael J., and Ulrich Strych. "Serratia marcescens and its extracellular nuclease." FEMS microbiology letters 165.1 (1998): 1-13. 3. Filimonova, Maria N., Kurt L. Krause, and Michael J. Benedik. "Kinetic studies of the Serratia marcescens extracellular nuclease isoforms." Biochemistry and molecular biology international 33.6 (1994): 122-1032. 4. Friedhoff, Peter, et al. "A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli." Protein expression and purification 5.1 (1994): 37-43. 5. Franke, Ingo, Gregor Meiss, and Alfred Pingoud. "On the Advantage of Being a Dimer, a Case Study Using the Dimeric Serratia Nuclease and the Monomeric Nuclease from Anabaena sp. Strain PCC 7120." Journal of Biological Chemistry 274.2 (1999): 825-832. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03695-10 |
|---|---|
| Supplier Part Number | Z03695-10 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |