Millipore Rlysozyme Solution, Liquid, -20deg C

Millipore Novagen rLysozyme Solution, Liquid Form, -20 deg C. rLysozyme solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3 to 5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster, BugBuster HT and PopCulture reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1 g cell paste with 45 to 60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27 to 33 KU/uL in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON X-100, pH 7.5. rLysozyme solution is stable at -20 deg C - Mill (Additional S&H or Hazmat Fees May Apply)