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Cell Signaling Ube1l2/Uba6 Antibody
List Price
$309.06
Your Price
$309.06
Cell Signaling Ube1l2/Uba6 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-13386S
MFG.PART:
13386S
UNSPSC:
12352202
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 117 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | UBE1L2/UBA6 Antibody recognizes endogenous levels of total UBE1L2/UBA6 protein. This antibody does not cross-react with UBE1/UBA1 or UBE1L/UBA7 proteins. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys810 of human UBE1L2 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thioester complex with the ubiquitin-activating enzyme (E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3).Ubiquitin-activating enzyme E1-like protein 2/Ubiquitin-like modifier-activating enzyme 6 (UBE1L2/UBA6) is ubiquitously expressed in human tissues and functions as an E1 enzyme related to UBE1/UBA1 (40% identity at the protein level). UBE1L2/UBA6 activates both ubiquitin and the ubiquitin-like protein FAT10 through a similar ATP dependent mechanism (4-6). Like other E1 protein family members, UBE1L2/UBA6 contains a conserved ATP-binding adenylation domain and an active site cysteine residue that are critical for enzymatic function (4,5). Research studies have demonstrated that UBE1L2/UBA6 expression is essential during the early stages of embryogenesis in mice (4). Furthermore, loss of neuronal UBE1L2/UBA6 expression promotes significant defects in neuronal structure and function, which contributes to a reduction in body weight and decreased postnatal viability (7). |
| SKU | CSIG-13386S |
|---|---|
| Supplier Part Number | 13386S |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |