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Cell Signaling Triad1 Antibody
Cell Signaling Triad1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$302.00
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$302.00
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NETA PART: | CSIG-13689S |
MFG.PART: | 13689S |
UNSPSC: | 12352202 |
Manufacturer: | Cell Signaling |
Size | 100 µl |
Reactivity | H M R Mk |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 58 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | TRIAD1 Antibody recognizes endogenous levels of total TRIAD1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with HHARI/ARIH1. |
Species Reactivity | Human, Mouse, Rat, Monkey |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRIAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | The E3 ubiquitin-protein ligase ARIH2 (TRIAD1) is an Ariadne subfamily ligase involved in the polyubiquitination of proteins designated for proteasomal degradation. The TRIAD1 nuclear protein contains an amino-terminal acidic region, a pair of RING fingers, two carboxyl-terminal coiled coil domains and a novel C6HC DRIL/IBR domain located between the RING fingers. Together, the paired RING fingers and DRIL/IBR domain form a highly conserved TRIAD (two RING fingers and DRIL) domain (1). Research studies suggest that TRIAD1 mediates both Lys48 and Lys63 protein polyubiquitination and acts as a negative regulator of myelopoiesis. TRIAD1 ubiquitin ligase inhibits myeloid cell proliferation by mediating protein ubiquitination through the ubiquitin-conjugating enzymes UbcH7 and UbcH13 (2,3). Experimental deletion of TRIAD1 in mice has a lethal effect, leading to death at the embryonic stage or later due to a severe, multi-organ inflammatory response. Results indicate that TRIAD1 binds IκBβ in dendritic cells and promotes the degradation of the NF-κB inhibitor (4). |
SKU | CSIG-13689S |
---|---|
Supplier Part Number | 13689S |
UM | UNIT |
UNSPSC | 12352202 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |