Revvity PhenoVe Fra-2 AM, Calcim Indicator

Revvity Calcim ion are imortant ignaling molecle mediating a road ectrm of intracelllar, intercelllar a well a extracelllar fnction. Ca2+ lay critical hyiological fnction ch a mcle contraction, neronal excitaility, cell migration and cell growth. Intracelllar Ca2+ i tored in the endolamic reticlm, arcolamic reticlm, and the mitochondria. Uon cell rface recetor timlation like GPCR, calcim i releaed from thee internal tore and it intracelllar concentration increae from 100 nM to aroximately 1 &m;M.Intracelllar Ca2+ reone have een widely invetigated with florecent calcim roe which florecence ignal increae on Ca2+ inding.The firt-generation of florecent calcim indicator, inclding Fra-2, Flo-3 and Rhod-2, were develoed, followed y a econd-generation, ch a Flo-4, with imroved roertie eecially rightne and ignal-to-noie ratio. However, the reence of anion tranorter exreed at the cell rface may imair the intracelllar retention of the florecent calcim indicator which are cleared ot from cell. Inhiitor of thee tranorter, ch a Proenecid, are commonly ed t ffer from limitation ch a cytotoxicity, off-target, or inconitent calcim flx kinetic. Sch limitation can e overcome with third generation of florecent calcim indicator inclding Cal-520, Cal-590 and their correonding Cal-520, Cal-590 Bright verion which do not reqire roenecid.PhenoVe Florecent Calcim Indicator, encomaing Fra-2 AM, Flo-4 AM, Cal-520 AM, Cal-590 AM, Cal-520 AM Bright and Cal-590 AM Bright, enale a myriad of alication a well a mltilexing caaility to nravel intracelllar calcim mediated fnction.PhenoVe Fra-2 AM i a high affinity cell ermeale Ca2+ indicator which i raidly metaolized y intracelllar eterae, leading to a green, florecent ignal.Featre:lliNmer of Vial Per Unit: 20liliQantity or Volme Per Vial: 50 µg (50 nmole)liliForm: SolidliliStorage: -16 °CliliRecommended Working Concentration: 5 µMliliMaximm Excitation Wavelength: 336 nm (Ca2+ free) 365 nm (Ca2+ ond)liliMaximm Emiion Wavelength: 505 nmliliLive Cell Staining: YeliliFixed Cell Staining: NolilFilter Set:lliExEm1 = 340510 nm ctoff 475 nmliliExEm2 = 380510 nm ctoff 475 nmlilEqivalent Nmer of Microlate:lli6-20 x 96-well microlatelili6-20 x 384-well microlatelili8-32 x 1536-well microlatelil-PEGSK(Additional S&H Or Hazmat Fees May Apply)