Revvity Pg-Seq Rapid V2 Kit

Fast PGT-A prep: 3 hours from cell lysis to NGS ready

Updated, streamlined, simple PGT protocolShape

From DNA to data, the PG-Seq^™ Rapid kit v2 includes all reagents required for cell lysis, whole genome amplification, indexing along with the PG-Find™ analysis software for automatic calling of aneuploidy and copy number variants.

Accurate Copy Number Detection

Extensively tested using over 100 cell lines and genomic DNA samples with known ploidy. Tested from whole chromosome aneuploidies down to segmental aberrations 7Mb in size with 30pg of genomic DNA or 5 cell fibroblast samples, representative of a trophectoderm biopsy. See PG-Seq^™ Rapid kit v2 app note for more information.

Flexible Kit Format

The PG-Seq^™ Rapid kit v2 has 48 rxns Two sets of 48 unique indexes are available, allowing up to 96 sample multiplexing (or up to 384 sample multiplexing available by custom request). Larger 40 µL WGA PCR 1 reaction volume enable options for alternative downstream processing. High WGA PCR 1 yield of 2-4 µg total DNA with only 23 cycles of PCR.

Improved whole genome coverage and accuracy

Enhanced PG-Find quality scores compared to previous versions of the kit signifies less noise, less bias, and higher confidence. Improved whole genome coverage when PG-Seq^™ Rapid v2 WGA PCR 1 product is used in hybridization capture panels.