Revvity HTRF Human Phospho-ATM Ser1981 Detection Kit, 500 Assay Points

This HTRF cell-based assay enables the rapid, quantitative detection of ATM phosphorylated at Serine 1981, as a readout of the ATM/CHK2 signaling pathway upon a DNA damage response (DDR). This kit is complementary to the phospho CHK2 Thr68 for studies of the ATM-CHK2 pathway.

In response to DNA damage, such as Double Strand Breaks (DSBs), the ATM€“Chk2 pathway and replication checkpoint responses are activated. These mediate G1/S checkpoints to arrest cell cycle progression and allow extra time for DNA repair.

In response to DNA DSBs, ATM autophosphorylates to generate the active monomeric kinase. Activation of ATM results in the phosphorylation of a diverse array of downstream targets such as H2A histone family member X, H2Ax, or the checkpoint kinase Chk2 and its downstream effectors.