Revvity Htrf Human And Mouse Lc3b-Ii Detection Kit, 500 Assay Points

Revvity lc3 is a mammalian homolog of the yeast atg8 protein and is distributed throughout mammalian tissues and cultured cells. lc3 is a key component of the autophagy process - a degradative program that maintains cellular homeostasis.during autophagy, cytoplasmic components such as cytosolic proteins and organelles are engulfed by autophagosomes concurrently, a cytosolic form of lc3 (lc3-i) is conjugated to phosphatidylethanolamine to form lc3-phosphatidylethanolamine conjugate (lc3-ii). this newly formed conjugate is recruited to autophagosomal membranes. the subsequent fusion of autophagosomes with lysosomes results in the formation of autolysosomes, and intra-autophagosomal components are degraded by lysosomal hydrolases. at the same time, lc3-ii within the autolysosomal lumen is degraded.the lc3-ii level has been found to be proportional to autophagosome abundance making lc3-ii the most popular marker of autophagy. - PEMSD (Additional S&H Or Hazmat Fees May Apply)