Revvity HTRF Human Total PINK1 Detection Kit, 500 Assay Points

The Total PINK1 assay is designed to monitor the cellular level of PINK1 protein.

PINK1 is a ser/thr kinase which plays a key role in PINK1-Parkin-mediated mitophagy. This signaling pathway is essential for toxic mitochondrial products removal maintaining cellular homeostasis. Dysregulation of this signaling is linked to neurodegenerative diseases, mainly Parkinsons disease.

In damaged depolarized mitochondria, PINK1 accumulates on the mitochondria outer membrane and activates PINK1-Parkin pathway by phosphorylating two substrates, the Ubiquitin-like domain of Parkin and Ubiquitin chains at position Ser65. Parkin is a Ubiquitin ligase E3 that conjugates Ubiquitin to impaired-mitochondrial proteins, leading to organelle degradation. The amplified PINK1/Parkin dependent Ubiquitin functions act as a signal for the sequestration and degradation of damaged mitochondria (mitophagy).

Depolarization of mitochondria by uncoupling agents, such as CCCP and valinomycin, causes PINK1 to accumulate on the outer mitochondrial membrane and trigger PhosphoS65-Ubiquitin production.