Revvity HTRF Human Total XBP1 Detection Kit, 10,000 Assay Points

The HTRF Human XBP1 detection assay monitors spliced XBP1 (XBP1s) as well as unspliced XBP1 (XBP1u), and is used to detect the expression of endogenous or overexpressed global XBP1 (XBP1s + XBP1u) in various cells.

Upon ER stress and accumulation of unfolded proteins, BiP releases its inhibitory binding to activate IRE1. IRE1 receptor dimerizes then autophosphorylates which activates its mRNase activity. Through this action, IRE1 splices XBP-1u mRNA to XBP1s, enabling its translation and then its translocation to the nucleus. XBP-1s then acts as a transcription factor.