Key Specifications Table
| Key Applications | Detection Methods |
|---|---|
| IHC, IF | Fluorescence |
| Description | |
|---|---|
| Catalogue Number | EF5-30A4 |
| Description | EF5 Hypoxia Detection Kit, Alexa Fluor® 488 |
| Background Information | A number of imaging markers which target hypoxia exist and are widely used in research. EF5 is a compound developed at the University of Pennsylvania by Dr. Cameron Koch and Dr. Sydney Evans. Upon injection into animal tissues, EF5 selectively binds to hypoxic cells and forms adducts. A mouse monoclonal antibody, clone ELK3-51 which is directly conjugated to Alexa Fluor® 488 is then used to selectively bind the EF5 adducts. Advantages of the EF5 Hypoxia Detection Kit: • EF5 exists in only one form: Pimidozole, an alternative hypoxia marker, exists in two forms; one of which is charged and very hydrophilic, the other lipophilic. Pimidozole thus has a very complex biodistribution. In contrast, EF5 is lipophilic and uncharged and this allows very rapid and even tissue distribution. • EF5 binding images can be calibrated to provide quantitative data on the pO2 values of each cell (Koch CJ, 2002): The fluorescent images obtained from EF5 binding can be calibrated according to camera settings and a “cube-binding” value which is obtained through a separate procedure. The intensity values of calibrated images are directly related to actual tissue pO2 values. As a result, these images provide information regarding not only where hypoxic areas may or may not be, but also data regarding the distribution and levels of hypoxia. Reference: Koch CJ (2002) Measurement of absolute oxygen levels in cells and tissues using oxygen sensors and 2-nitroimidazole EF5. Methods in Enzymology 352: 3-31. |
| Product Information | |
|---|---|
| Components |
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| Detection method | Fluorescence |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The EF5 Hypoxia Detection Kit provides a sensitive and quantitative method to detect and measure tissue hypoxia in animal and human tumors, normal tissues and cells. |
| Key Applications |
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| Application Notes | Antibody is provided in a concentrated format at 2 mg/mL. For optimal performance, antibody should be diluted to a working concentration of at least 75 ug/mL. Refer to product datasheet for details. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Store kit at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 1 Kit |