The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore This Anti-Phospho Histone H2a.X (Ser139) Antibody, Clone Jbw301, Alexa Fluor® 488 Conjugate Is Validated For Use In Icc For The Detection Of Phospho Histone H2a.X (Ser139). - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.
Product Information
Format
AlexaFluor®488
Presentation
Purified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% Sodium Azide.
Quality Level
MQ100
Applications
Application
This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Key Applications
Immunocytochemistry
Biological Information
Immunogen
Linear peptide corresponding to phospho-Histone H2A.X (Ser139).
Clone
JBW301
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Vertebrates based on 100% sequence homology.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP002096
Gene Symbol
H2AFX
H2AX
Purification Method
Protein G Purified
UniProt Number
P16104
Molecular Weight
17 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H2A.X (Ser139) in Staurosporin treated HeLa cells, and not in untreated HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
