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Millipore This Anti-Phospho-Gab1 (Tyr627) Antibody Is Validated For Use In Wb For The Detection Of Phospho-Gab1 (Tyr627). - Mill (Additional S&H or Hazmat Fees May Apply)
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Quality Level
MQ100
Applications
Application
This Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).
Key Applications
Western Blotting
Application Notes
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Gab1 (Tyr627) in RIPA lysate of PDGF treated NIH3T3 cells.
Biological Information
Immunogen
Peptide corresponding to amino acid region encompassing the human and mouse phospho-Gab1 (Tyr627)
Host
Rabbit
Specificity
Gab1 phosphorylated on Tyrosine residue 627.
Isotype
IgG
Species Reactivity
Mouse
Species Reactivity Note
Predicted to cross-react with human based on 100% sequence homology, but have not been tested.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_002039.2
NM_207123.1
Entrez Gene Summary
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. The encoded protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
GAB1
Modifications
Phosphorylation
Purification Method
Affinity Purfied
UniProt Number
Q13480
UniProt Summary
FUNCTION: SwissProt: Q13480 # Probably involved in EGF and insulin receptor signaling. SIZE: 694 amino acids; 76616 Da SUBUNIT: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. PTM: Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. SIMILARITY: SwissProt: Q13480 ## Belongs to the GAB family. & Contains 1 PH domain.
Molecular Weight
115 kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
