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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-850 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | 100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-LIMK 1/2 (Tyr507/Thr508) using this anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody validated for use in WB. |
| Key Applications |
|
| Application Notes | Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated LIMK 1/2 (Tyr507/Thr508) in RIPA lysates of SDF-1α treated Jurkat cells. |
| Biological Information | |
|---|---|
| Immunogen | Peptide corresponding to amino acid region encompassing the human, mouse, rat and chicken phospho-LIMK1/2 (Tyr507/Thr508) |
| Host | Rabbit |
| Specificity | Recognizes phosphorylated LIMK1/2 (Tyr507/Thr508) |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Predicted to cross-react with Mouse, Rat, and Frog (100% homologous) based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| Modifications |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P53671 # Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. SIZE: 638 amino acids; 72232 Da SUBUNIT: Binds ROCK1 and LKAP. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. TISSUE SPECIFICITY: Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. PTM: Phosphorylated on serine and/or threonine residues by ROCK1. SIMILARITY: SwissProt: P53671 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 2 LIM zinc-binding domains. & Contains 1 PDZ (DHR) domain. & Contains 1 protein kinase domain. |
| Molecular Weight | 72 kDa (LIMK1) and 65 kDa (LIMK2) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |