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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-143 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Brk Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | 50 µg of affinity purified rabbit polyclonal IgG in 0.02 M PBS, 250 mM sodium chloride containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Brk using this Anti-Brk Antibody validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH conjugated synthetic peptide corresponding to the N-terminal region of human Brk. |
| Host | Rabbit |
| Specificity | Recognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. |
| Gene Symbol |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression. SIZE: 451 amino acids; 51834 Da SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors. TISSUE SPECIFICITY: Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme. SIMILARITY: SwissProt: Q13882 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. |
| Molecular Weight | 52 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store for 1 year at 2-8°C from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |