| Description | |
|---|---|
| Catalogue Number | 12-414 |
| Brand Family | Upstate |
| Trade Name |
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| Description | CHKtide, biotin conjugate |
| Product Information | |
|---|---|
| Presentation | sterile, distilled water |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Biological Information | |
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| Entrez Gene Number |
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| Gene Symbol |
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| Protein Target | CHK |
| Target Sub-Family | CAMK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14757 # Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X- S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at Ser-178 and Thr-507 and phosphorylation of CDC25C at Ser-216 creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at Ser-76, Ser-124, Ser-178, Ser-279 and Ser- 293 promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK- cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at Thr-309, which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at Ser-743, which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. SIZE: 476 amino acids; 54420 Da SUBUNIT: Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B. TISSUE SPECIFICITY: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. DOMAIN: SwissProt: O14757 The autoinhibitory region (AIR) inhibits the activity of the kinase domain. PTM: Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity. & Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity). SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated as a substrate for CHK enzymes. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
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| Material Size | 200 µg |