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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, M, R | IHC, WB | Gt | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB144 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Choline Acetyltransferase Antibody |
| Alternate Names |
|
| Background Information | Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Goat antisera, liquid, no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Choline Acetyltransferase Antibody is an antibody against Choline Acetyltransferase for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunoblot: 1:1,000-1:5,000 Immunohistochemistry: 1:500-1:1,000. See page two of this data sheet. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human placental enzyme. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Goat |
| Specificity | Recognizes cholinergic neurons in brain and central nervous systems. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5 untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family. |
| Molecular Weight | 70 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |