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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, ICC, IHC, RIA | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2033 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Fibronectin Antibody |
| Background Information | Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues. Radioimmunoassay: A previous lot of this antibody was used in RIA. ELISA: A 1:200 dilution of a previous lot was used in ELISA. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Fibronectin extracted and purified from mouse plasma. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by immunocytochemistry on NIH/3T3 cells. Immunocytochemistry: Representative image from a previous lot. Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488® -Phalloidin (Green). Nucleus is stained with DAPI (Blue). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |