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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IHC | Gp | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5898 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Protein Gene Product 9.5 Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Liquid. Contains 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:500. Optimal working dilutions must be determined by end user. blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. |
| Biological Information | |
|---|---|
| Immunogen | A 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Guinea Pig |
| Specificity | Recognizes Protein Gene Product 9.5 (PGP9.5). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P09936 # Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. SIZE: 223 amino acids; 24824 Da SUBUNIT: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. PTM: O-glycosylated (By similarity). DISEASE: SwissProt: P09936 # Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. SIMILARITY: SwissProt: P09936 ## Belongs to the peptidase C12 family. MISCELLANEOUS: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |