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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Mk, R | IHC, WB | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1585 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
|
| Presentation | UnPurified mouse tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:5-1:125 Western blot: 1:50-1:100 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995). |
| Epitope | POU-domain protein |
| Clone | 5A3.2 |
| Host | Mouse |
| Specificity | Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages (Gerrero et al., 1993 [PubMed 8248179]).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q01851 # Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. SIZE: 423 amino acids; 42940 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye. DEVELOPMENTAL STAGE: Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth. SIMILARITY: SwissProt: Q01851 ## Belongs to the POU transcription factor family. Class- 4 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain. |
| Molecular Weight | 46 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |