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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk, Rb | ELISA, IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1922Z |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Laminin α2 Antibody, clone 5H2, Azide Free |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Laminin α2 Antibody, clone 5H2, Azide Free detects level of Laminin α2 & has been published & validated for use in ELISA, IH, IP & WB. |
| Key Applications |
|
| Application Notes | ELISA: 50% maximal binding to human merosin at 1:50,000. Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody. Immunofluorescence Affinity chromatography Immunoprecipitation Immunoblotting Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified human merosin. |
| Clone | 5H2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the 80 kDa fragment of the M-chain of human merosin. SPECIES REACTIVITIES: Cross reacts with monkey and rabbit merosin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P24043 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. SIZE: 3110 amino acids; 342771 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (merosin) and laminin-4 (S- merosin). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. TISSUE SPECIFICITY: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone. DOMAIN: SwissProt: P24043 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular. DISEASE: SwissProt: P24043 # Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI. SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain between 2 and 8°C for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |