Cell Signaling Ras (G12d Mutant Specific) (D8h7) Rabbit mAb

Name: Cell Signaling Ras (G12d Mutant Specific) (D8h7) Rabbit mAb
SKU: CSIG-14429S
Price: 480.76 USD

List Price $480.76 Your Price $480.76

Cell Signaling Ras (G12d Mutant Specific) (D8h7) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-14429S

MFG.PART: 14429S

UNSPSC: 41116133

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	21
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Ras (G12D Mutant Specific) (D8H7) Rabbit mAb recognizes endogenous levels of Ras G12D mutant protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to G12D mutant sequence of human Ras protein.
Background	The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

Specifications