LI-COR IRDye® 680lt Goat Anti-Rabbit Igg Secondary Antibody, 0.5 mg

Immunogen

Rabbit IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled rabbit IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

• Western Blot
• Protein Array
• Immunohistochemistry
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging

Not Recommended for:

• Small Animal Imaging
• In-Cell Western Assay
• On-Cell Western Assay

Formulation

IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 925-68021: RRID AB_2713919
• P/N 926-68021: RRID AB_10706309

Example Data