Sigma-Aldrich Ammonium Test Mcolortesttm

Specific Analyte(S)

ammonium

Quality Level

100

Compatibility

for use with MQUANT^®

Detection Method

colorimetric

Storage Temp.

15-25°C

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Colorimetric & Titrimetric Test Kits

General Description

Method: colorimetric, indophenol blue, with color card and comparator block with long test tubes 0.025-0.050-0.075-0.10-0.15-0.20-0.25-0.30-0.40 mg/l NH<SUB>4</SUB> 0.02-0.04-0.06-0.08-0.12-0.16-0.19-0.23-0.31 mg/l NH4-N MQuant^®With the MQuant^®Ammonium test (formally MColortest^®) ammonium ions can be detected in e.g. groundwater and surface water, seawater, drinking water and aquarium water. Ammonium nitrogen (NH4-N) occurs partly in the form of ammonium ions and partly as ammonia. A pH-dependent equilibrium exists between the two forms.

Method
In strongly alkaline solution ammonia reacts with a chlorinating agent to form monochloramine. This in turn reacts with thymol to form a blue indophenol derivative. Due to the intrinsic yellow coloration of the reagent blank, the measurement solution is yellow-green to green in color. The ammonium concentration is measured semiquantitatively by visual comparison of the color of the measurement solution with the color fields of a color card.The unique brilliance of the print and their fine color graduation allow precise analyses, even at the lower end of the concentration range. Due to the long test tubes a sensitive measurement range can be covered.

This test is suitable for ammonium determination in

• Groundwater and surface water
• Drinking water and mineral water
• Wastewater
• Aquarium water, waters from aquaculture
• Boiler and boiler feed water, cooling water
• Swimming-pool water
• Nutrient solutions for fertilization
• Food after appropriate sample pretreatment

This test is not suited for seawater.

Application

• Evaluation of Water Ammonium Ion Test Kit and Its Feasibility for the Analysis of Protein in Food.: This study assesses a water ammonium ion test kit′s application in food protein analysis, highlighting its potential benefits for ensuring food safety and quality (Wu et al., 2022).


• Technical note: Evaluation of a colorimetric point-of-care test for measuring urine ammonium concentration in periparturient dairy cattle.: Discusses a colorimetric point-of-care test for measuring urine ammonium in dairy cattle, crucial for animal health monitoring and management (Megahed & Constable, 2020).

MCOLORTEST is a registered trademark of Merck KGaA, Darmstadt, GermanyMQUANT is a registered trademark of Merck KGaA, Darmstadt, GermanyGHS02,GHS05,GHS07H225 - H290 - H302 - H314 - H336 - H412P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338