Sigma-Aldrich Phosphate Test Method: Col

Specific Analyte(S)

phosphate

Quality Level

100

Measuring Range

1.5-100 mg/L (PO₄-P)

Compatibility

for use with MQUANT^®

Detection Method

colorimetric

Storage Temp.

15-25°C

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Colorimetric & Titrimetric Test Kits

Phosphates

General Description

Method: colorimetric, VM, with color-disk comparator 1.5 - 3 - 6 - 9 - 12 - 16 - 20 - 40 - 100 mg/l PO₄-P MQuant^®The MQuant^®Phosphate test (formally MColortest^®) can be used for the determination of orthophosphate in:

• Groundwater and surface water, seawater
• Drinking water and mineral water
• Waters from aquaculture
• Industrial water
• Boiler water
• Process water
• Wastewater
• Electroplating wastewater
• Nutrient solutions for fertilization
• Soils after appropriate sample pretreatment

Method
In sulfuric solution orthophosphate ions react with ammonium vanadate and ammonium heptamolybdate to form orange-yellow molybdovanadophosphoric acid. The phosphate concentration is measured semiquantitatively by visual comparison of the color of the measurement solution with the color fields of a color disk. The test evaluates the color reaction based on the transmitted light method. So even turbid and colored water samples can be analyzed without further preparation.
The color disk is made of very durable, lightfast plastic, thus suitable for industrial areas and wet environments.
Using the refill pack 1.18466 containing all the reagents, the original test kit can be used for a very long time, making it a particularly economical and ecological system.

Application

• Environmental Monitoring: Phosphate tests are extensively used in environmental monitoring to assess the levels of phosphate in water bodies. These tests help in evaluating the impact of agricultural runoff, industrial discharge, and wastewater treatment on water quality. Recent studies have focused on using selective colorimetric nano-sensing solutions to determine phosphate ions in drinking water samples, highlighting their importance in ensuring safe and clean water supplies (Salem and Draz, International Journal of Environmental Analytical Chemistry, 2020).

MCOLORTEST is a registered trademark of Merck KGaA, Darmstadt, GermanyMQUANT is a registered trademark of Merck KGaA, Darmstadt, GermanyGHS05H290 - H314P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363