Key Specifications Table
| Key Applications | NMWL | Volume | Minimum Final Concentrate Volume |
|---|---|---|---|
| PExt, Protein Purification | 30 KDa | 0.5 mL | 15 µL |
| Description | |
|---|---|
| Catalogue Number | UFC503008 |
| Replaces | 42422 |
| Trade Name |
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| Description | Amicon Ultra-0.5 Centrifugal Filter Unit |
| Background Information | AMICON ULTRA 0.5ML - 30KDa cutoff. Available in 4 pack sizes. UFC503008 - 8 pack UFC503024 - 24 pack UFC503096 - 96 pack UFC5030BK - 500 pack Concentrates 500 µL down to 15 µL Concentration factor 25 x to 30 x High sample recovery (greater than 90% of dilute starting solution) Fast processing time - 10 to 30 minutes Reverse spin capability provides consistent recoveries Applications: Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms Purification of macromolecular components found in tissue culture extracts or cell lysates Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC Desalting, buffer exchange and protein dialysis Material Supplied: The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. Performance – DNA Concentration: The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA (TE) buffer are recommended. |
| Product Information | |
|---|---|
| Filter Code | PLCGC |
| Color Code | Clear |
| Filter Type | Ultrafiltration |
| NMWL | 30 KDa |
| Quality Level | MQ400 |
| Applications | |
|---|---|
| Application | Ultracel-30 regenerated cellulose membrane, 0.5 mL sample volume |
| Key Applications |
|
| Biological Information | |
|---|---|
| Sterility | Non-Sterile |
| Physicochemical Information | |
|---|---|
| Centrifugal Application | Protein Concentration and Desalting |
| Dimensions | |
|---|---|
| Length | 4.99 cm (1.96 in.) |
| Diameter | 10.8 mm |
| Filtration Area | 1 cm² |
| Minimum Final Concentrate Volume | 15 µL |
| Volume | 0.5 mL |
| Materials Information | |
|---|---|
| Chemistry |
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| Device Material |
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| Packaging Information | |
|---|---|
| Material Size | 8 |