Takara Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version) \Each

Takara The Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of two genes of interest from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI Vector Set, consisting of a bidirectional vector into which both genes of interest can be cloned and simultaneously expressed, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS. - TAKBGN (Additional S&H or Hazmat Fees May Apply)