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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT188 |
| Description | Anti-Dynamin-1/2 Antibody, clone Hudy-1 |
| Alternate Names |
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| Background Information | Dynamin-1 & Dynamin-2 belong to the dynamin superfamily of large GTPases. Dynamins are multidomain proteins consisting of a GTP-binding domain, a middle domain, and a GTP-effector domain. Dynamins also contain a pleckstrin homology (PH) domain which may facilitate membrane binding. Dynamin-1 is highly abundant in the brain particularly at synaptic contacts, whereas Dynamin-2 is ubiquitously expressed in non-neuronal cells. Dynamins are involved in receptor-mediated endocytosis of clathrin-coated vesicles. Dynamin-1 interacts with an array of cellular targets via its SH3 binding domain and is implicated in late stages of vesicle formation by forming oligomers around the “neck” of budding vesicles and inducing membrane fission; it may also play a role in cytoskeleton rearrangement and cytoskinesis. Dynamin-2 is involved in the formation of clathrin-coated vesicles but may also be involved in the recycling of receptors between endosomes and the trans-Golgi network. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot was used by an independent laboratory in HeLa cells (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.) |
| Biological Information | |
|---|---|
| Immunogen | Recombinant full length human Dynamin-1 expressed in transfected Sf9 cells. |
| Clone | Hudy-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody detects both Dynamin-1 and Dynamin-2. (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.) |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3 region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. CATALYTIC ACTIVITY: GTP + H2O = GDP + phosphate. SUBUNIT STRUCTURE: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity). Interacts with PHOCN By similarity. Interacts with SNX9. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated. SEQUENCE SIMILARITIES: Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain. |
| Molecular Weight | ~97 kDa observed. Dynamin 1 has a calculated MW of 97 kDa. Dynamin 2 has a calcluated MW of 98 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in rat brain tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin-1/2 on 10 µg of rat brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |