| Description | |
|---|---|
| Catalogue Number | 14-377 |
| Brand Family | Upstate |
| Trade Name |
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| Description | MKK4/SKK1 Protein, active, 10 µg |
| Overview | N-terminal GST tagged, murine MKK4 residues 34-end |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal GST tagged, murine MKK4 residues 34-end, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in E. coli |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. |
| Gene Symbol |
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| Protein Target | MKK4 |
| Purification Method | Purified using glutathione agarose. Activated by incubation with MEKK and then re-purified using Ni2+/NTA agarose |
| Target Sub-Family | STE |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). SIZE: 399 amino acids; 44288 Da SUBUNIT: Interacts with SPAG9 (By similarity). TISSUE SPECIFICITY: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity). SIMILARITY: SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 67.7kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of ATF2 substrate |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-377M when ordering the 250 µg size. |