| Description | |
|---|---|
| Catalogue Number | 14-451 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | ROKα/ROCK-II Protein, active, 10 µg |
| Overview | N-terminal His6-tagged recombinant human ROCK-II residues 11-552 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Source | expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. |
| Gene Symbol |
|
| Protein Target | ROKα/ROCK-II |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q13464 # Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere- dependent exit from mitosis (By similarity). SIZE: 1354 amino acids; 158175 Da SUBUNIT: Binds RHOA that has been activated by GTP binding. Binds ADD1, GEM, RHOB, RHOC, myosin light chain and VIM (By similarity). Binds RHOE, PPP1R12A (MYPT1/MBS), LIMK1 and LIMK2. SUBCELLULAR LOCATION: Cytoplasm. Centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes. TISSUE SPECIFICITY: Detected in blood platelets.DOMAIN:SwissProt: Q13464 The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated. PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. & Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 REM (Hr1) repeat. MISCELLANEOUS: Inhibited by Y-27632. |
| Molecular Weight | 63.3kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size (14-451M), please inquire for pricing and availability. |