| Description | |
|---|---|
| Catalogue Number | 14-500 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Flt-3 Protein, active, 10 µg |
| Overview | N-terminal GST tagged, recombinant human Flt3, amino acids 564 - end |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal GST tagged, recombinant human Flt3, amino acids 564 -end, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. |
| Gene Symbol |
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| Protein Target | Flt3 |
| Purification Method | Glutathione Sepharose™ |
| Target Sub-Family | TK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P36888 # Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. SIZE: 993 amino acids; 112903 Da SUBUNIT: Interacts with FIZ1 following ligand activation (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Bone marrow cells. SIMILARITY: SwissProt: P36888 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 1 protein kinase domain. |
| Molecular Weight | 77.4kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of Abltide |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-500M when ordering the 250μg size. |