| Description | |
|---|---|
| Catalogue Number | 14-505 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PKC iota Recombinant Human Protein, Active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant full-length human PKC iota PKC Lipid activator (Catalog # 20-133) is required for use. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant full-length human PKC iota. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
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| Source | Human PKC iota expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. |
| Gene Symbol |
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| Protein Target | PKCι |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P41743 # Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NFkappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2 and GAPDH and recruitment to vesicular tubular clusters (VTCs). SIZE: 587 amino acids; 67258 Da SUBUNIT: Forms a complex with SQSTM1 and MP2K5 (By similarity). Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts with PARD6A, PARD6B and PARD6G. Part of a quaternary complex containing aPKC, PARD3, a PARD6 protein (PARD6A, PARD6B or PARD6G) and a GTPase protein (CDC42 or RAC1). Part of a complex with LLGL1 and PARD6B. Interacts with CENTA1. Interaction with SMG1, through the ZN-finger domain, activates the kinase activity. Interacts with CDK7. Forms a complex with RAB2 and GAPDH involved in recruitment onto the membrane of vesicular tubular clusters (VTCs). SUBCELLULAR LOCATION: Cytoplasm. Membrane. Endosome. Nucleus. Note=Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with Rab2. TISSUE SPECIFICITY: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers. DOMAIN: SwissProt: P41743 The OPR domain mediates interaction with SQSTM1 (By similarity). & The C1 domain does not bind diacylglycerol (DAG). PTM: On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-256, Tyr-271 and Tyr-325. Phosphorylation on Tyr-256 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-325 is important for NF-kappaB stimulation. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 OPR domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. |
| Molecular Weight | 68.6kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by phosphorylation of PKC Substrate Peptide |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-505M when ordering the 250 µg size. |