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N-terminal his6-tagged recombinant full-length human Lyn
Product Information
Quality Level
MQ100
Applications
Application
Active, N-terminal his6-tagged recombinant full-length human Lyn, for use in Kinase Assays.
Key Applications
Kinase Assay
Application Notes
Kinase Assays: Depending upon the specific activity of the lot , 7.6 -15.3ng of enzyme phosphorylated 0.1 mg/ml poly (Glu, Tyr) 4:1 {Sigma P7244} in a standard kinase assay. See technical datasheet included with purchased product for lot specific activity values.
Biological Information
Source
expressed in Sf21 cells
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_002350.1
Gene Symbol
LYN
JTK8
FLJ26625
Protein Target
Lyn
Purification Method
Ni2+/NTA-agarose
Target Sub-Family
TK
UniProt Number
P07948
UniProt Summary
SIZE: 512 amino acids; 58574 Da SUBUNIT: Interacts with phosphorylated LIME1 and with CD79A upon BCR activation. Interacts with Epstein-Barr virus LMP2A. Interacts with TGFB1I1. TISSUE SPECIFICITY: Expressed in primary neuroblastoma tumors. SIMILARITY: SwissProt: P07948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight
59.6kDa
Product Usage Statements
Quality Assurance
routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
6 months at -70°C
Packaging Information
Material Size
10 µg
Material Package
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-510M when ordering the 250 µg size.
