| Description | |
|---|---|
| Catalogue Number | 14-518 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PKCε Protein, active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant PKCε, amino acids 2-end PKC Lipid activator (Catalog # 20-133) is required for use. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant PKCε, amino acids 2-end. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Human PKCε expressed in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. |
| Gene Symbol |
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| Protein Target | PKCε |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q02156 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. SIZE: 737 amino acids; 83674 Da SUBUNIT: Forms a ternary complex with TRIM63 and GN2BL1 (By similarity). DOMAIN: SwissProt: Q02156 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. PTM: Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. |
| Molecular Weight | 84.6kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by phosphorylation of PKC Substrate Peptide |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |
| Material Package | 2 vials at 5μg each Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-518M when ordering the 250μg size. |