| Description | |
|---|---|
| Catalogue Number | 14-519 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Cdk6/Cyclin D3 Protein, active, 10 µg |
| Overview | N-terminal, His6-tagged fulllength human cdk6 complexed with N-terminal GSTtagged full-length human cyclin D3 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal, His6-tagged full length human Cdk6 complexed with N-terminal GST-tagged full-length human Cyclin D3, for use in Kinase Assays. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Source | expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. |
| Gene Symbol |
|
| Protein Target | CDK6/cyclinD3 |
| Purification Method | Glutathione-agarose |
| Target Sub-Family | CMGC |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q00534 # Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. SIZE: 326 amino acids; 36938 Da SIMILARITY: SwissProt: Q00534 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 38kDa (cdk6) and 59kDa (cyclin D3) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of histone H1 |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size as catalog # 14-519M - Call for pricing and availability. |