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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, IHC, IP, RIA, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1864 |
| Replaces | 04-1117 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tubulin Antibody, clone YL1/2 |
| Background Information | Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Tubulin using this Anti-Tubulin Antibody, clone YL1/2 validated for use in ELISA, IH, IP, RIA & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:50-1:100 dilutoin from a previous lot was used on frozen sections. ELISA: 1:100-1:1,000 dilution from a previousl lot was used. Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation. Radioimmunoassy: A previous lot of this antibody was used on Radioimmuoassy. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Yeast tubulin |
| Epitope | The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). |
| Clone | YL1/2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SUBUNIT STRUCTURE: Dimer of alpha and beta chains. TISSUE SPECIFICTY: Expressed at a high level in fetal brain. Ref.7 PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively By similarity. DISEASE: Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem. Ref.7 SIMILARITY: Belongs to the tubulin family. |
| Molecular Weight | 55 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on A431 lysates. Western Blot Analysis: 1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |