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Sartorius Sartobind Lab Affinity.Ida 75
List Price
$256.00
Your Price
$256.00
Sartorius Sartobind Lab Affinity.Ida 75 - SAT
NETA PART:
SAT-93IDA-42DB-12-V
MFG.PART:
93IDA-42DB-12--V
UNSPSC:
41115700
Manufacturer:
Sartorius
Based on the Patented Sartobind® Platform
Sartobind® Lab membrane adsorbers for immobilized metal affinity chromatography (IMAC) are ready-to-use units in a syringe filter format. They are based on the patented macroporous Sartobind® membranes, with iminodiacetic acid (IDA) covalently bound to the entire internal surface of the pores. This results in a chromatography matrix with superior flow rates and high binding capacity. The IDA ligand enables coupling of the metal ion of your choice to the membrane adsorber, ensuring simple process optimization for maximum purity and yield of each his-tagged protein.
Ultimate Versatility
Sartobind® Lab IDA units are available with a 2.1 mL bed volume, providing binding capacities up to 7.5 mg his-tagged protein per unit. Equipped with standard Luer lock connectors, Sartobind® Lab units enable simple, equipment-free handling by syringe, or can be easily connected to FPLC systems for automated purifications. The units are compatible with most common chromatography buffers and can be regenerated for multiple purification cycles, ensuring high-cost efficiency.
Applications
Application - Screening
- Optimization
- His-tagged Protein Purification
- Screening
Intended Use Research Use Only (RUO)
Dimensions
Bed Height 4 mm Membrane Area 75 cm²
Features
Reusable Yes
Materials of Construction
Housing Material Polypropylene (PP) Membrane Material Stabilized reinforced cellulose Reinforcement Material Polyester
Operating Conditions
Maximum Operating Pressure 0.6 MPa Recommended Flow Rate 10-63 mL/min (5-30 MV/min) pH Stability pH 1 – 12 (short term), pH 3 – 9 (long term)
Physicochemical Information
Bed Volume 2.1 mL Chemical Compatibility Stable in common chromatography buffers, avoid peroxide and other oxidizing or reactive reagents Ligand Iminodiacetic acid (IDA) Ligand Density 5 µeq/cm² Pore Size 3 - 5 µm Recommended Chelating Ions Ni2+, Co2+, Cu2+, or Zn2+ Reference Protein Polyhistidine tagged protein Typical Dynamic Binding Capacity 10% per unit 7.5 mg Void Volume 1.3 mL
Product Information
Brand Sartobind® Lab Membrane Type Metal chelate Pack Size 2 What you receive - 2 units
- 1 operating instruction sheet
- 2 units
| SKU | SAT-93IDA-42DB-12-V |
|---|---|
| Supplier Part Number | 93IDA-42DB-12--V |
| UM | PK |
| UNSPSC | 41115700 |
| Manufacturer | Sartorius |
| CountryOfOrigin | Germany |
| ProductLine | SAT |
| Qty | 2 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 4 |
| Hazardous | N |