Sigma-Aldrich Silica Gel 60 F254 1 Tlc A

Material

aluminum support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 1 roll

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Roll L × W

500 cm × 20 cm

Particle Size

10-12 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

1 Aluminium roll 500 x 20 cmOur classical silica TLC plates are based on a combination of our proven silica gel 60 and the addition of a unique polymeric binder resulting in a very adherent and hard surface that will not crack or blister and even allow writing with a pensil on the surface without risk to damage the layer.

Application

• Determination of β-blocking receptor drugs in silica gel TLC systems with the mobile phase containing surfactant.: This paper investigates the use of silica gel TLC plates with surfactant-containing mobile phases for the separation and quantitative analysis of β-blocking receptor drugs. The environmentally friendly method provides accurate LOD and LOQ values for various drugs (Traczuk and Polak, 2022).


• Separation of peptides in thin-layer chromatography systems with C18 silica-based adsorbents.: This paper investigates the separation of peptides using thin-layer chromatography (TLC) systems with C18 silica-based adsorbents, including Silica gel 60 F₂₅₄plates. The study demonstrates the utility of these systems in peptide analysis (Gwarda et al., 2016).

Linkage

Replaces: 5562-7; 5562

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 160 - 210 µm
Deviation of layer thickness per plate: ≤ 40 µm
Chromatographic testing:
Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
11 - 25
- Ceres Black G, colour test, lipophile: 34 - 48
- ceres Violet brn, colour test, lipophile: 52 - 67
Typical value determined on a conditioned sheet
Eluent: toluene (45% rel. humidity)