Sigma-Aldrich Cellulose 25 Tlc Plastic S

Material

cellulose matrix
plastic support

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator: no

Packaging

pkg of 25 sheets

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

70-110 μm

Sheet L × W

20 cm × 20 cm

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Plastic sheets 20 x 20 cmOur Cellulose plates are used to analyze polar substances. An organic sorbent, cellulose is perfect for separating hydrophilic substances by partition chromatography. Typical applications include the analysis of amino acids, carbohydrates, and phosphates as well as nucleic acid and nucleic acid derivatives. We offer cellulose plates in two grades: TLC for conventional and HPTLC for demanding, high-performance separations.

Application

• A colorimetric method for the enzymatic analysis of gases: the determination of ethanol and formaldehyde vapors using solid alcohol oxidase.: This study presents a novel colorimetric method utilizing solid alcohol oxidase for the determination of ethanol and formaldehyde vapors. The technique, employing cellulose TLC plastic sheets, provides an efficient and accurate means of gas analysis in various environments (Barzana et al., 1989).

Linkage

Replaces: 5577-7; 5577

Analysis Note

Layer thickness: 70 - 110 µm
Deviation of layer thickness per plate: ≤ 30 µm
Aminoacid test
hRf-values
- L-serine, amino acid test:
20 - 30
- L-threonine, amino acid test: 29 - 36
- L-alanine, amino acid test: 30 - 40
- L-methionine, amino acid test: 49 - 60
- L-phenylalanine, amino acid test: 59 - 70
- L-leucine, amino acid test: 66 - 76
Typical value determined on a sheet
Eluent: 1-Butanol/ acetone/ acetic acid/ water (35/35/7/23 v/v/v/v) twice developed