Sigma-Aldrich Silica Gel F254 Glp 25 Hpt

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Plate L × W

20 cm × 10 cm

Particle Size

5-6 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 20 x 10 cmThese plates feature individual laser coding for GLP applications. Designed for GLP applications, the tops of each of these plates bear an item, batch, and individual plate number. As a result, lab professionals can easily record and archive every plate they use. Based on our proven silica found in TLC and HPTLC plates, these GLP plates deliver equally unsurpassed separation performance.

Application

Glass HPTLC Silica gel F₂₅₄ GLP plates. Silica gel HPTLC plates size 20 x 10 cm, 25 sheets.

Linkage

Replaces: 5613-6; 5613

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steriod test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)